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产品编号 | KMP2793 |
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产品名称 | Human TIGIT Protein, mFc Tag |
产品描述 | The Human TIGIT Protein(KMP2793) is produced in HEK293 Cells. The target gene encoding the human TIGIT (NP_776160.2) (Met1-Pro141) is expressed with the Fc region of mouse IgG2a at the C-terminus. |
分子名称 | TIGIT |
产品别称 | T细胞免疫球蛋白, Human TIGIT Protein, mFc Tag, TIGIT |
物种 | Human |
宿主 | HEK293 Cells |
规格 | 50ug, 100ug, 200ug |
纯度 | >95% as determined by SDS-PAGE |
纯化方式 | Affinity purification |
内毒素水平 | <1.0 EU/ug determined by the LAL method |
缓冲体系 | PBS, pH7.4 |
产品背景 | TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition. |
保存条件 | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
说明 | This product is for research use only. |